Anxiety disorders are a prevalent mental health concern, for which current anxiolytic medications help to alleviate symptoms of, though often coincide with unwanted side effects. The present study investigated the relationship between cannabidiol (CBD) ingestion (in mL) and anxiety in longitudinal baseline conditions as well the effect in an experimental manipulation, measured using a mood scale and by heart rate. It was hypothesized that CBD ingestion would lead to a decrease in both self-reported and physiological anxiety. The results supported the hypotheses. These results are discussed in terms of the important implication that CBD does appear to have an anxiolytic effect, possibly without the harmful side effects seen in other interventions.