In this research paper, we aim to gain a better understanding of the biological functions that occur when an individual takes part in physical activity, especially in regard to their stress and anxiety levels. Previous research has shown that physical activity can increase serotonin levels, decrease amygdala activity, and increase irisin levels. We measured physical activity by minutes of exercise, rated serotonin levels through number of minutes slept, determined amygdala activity from heart rate after viewing scary stimuli, and inferred irisin levels after measuring Body Mass Index (BMI) each day. Our correlational study results showed that the serotonin levels, amygdala activity, and irisin levels were not significantly correlated with physical activity levels.